Mouse embryonic chimeras: tools for studying mammalian development.
نویسندگان
چکیده
Embryonic chimeras of the mouse are well-established tools for studying cell lineage and cell potential. They are also a key part of the analysis of complex phenotypes of mutant mice. By combining embryonic stem cell technology, molecularly tagged mutations and sensitive cell lineage markers, chimeras can provide invaluable insights into the tissue-specific requirement and the mode of action of many mouse genes.
منابع مشابه
"Interspecific Chimeras in Mammals: Successful Production of Live Chimeras Between Mus musculus and Mus caroli" (1980), by Janet Rossant and William I. Frels
In 1980 Janet Rossant [5] and William I. Frels [6] published their paper, ?Interspecific Chimeras in Mammals: Successful Production of Live Chimeras Between Mus musculus and Mus caroli ,? in Science. Their experiment involved the first successful creation of interspecific mammalian chimeras [7]. Mammalian chimeras [8] are valuable for studying early embryonic development. However, in earlier st...
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The developmental potential of embryonic stem (ES) cells versus 3.5 day inner cell mass (ICM) was compared after aggregation with normal diploid embryos and with developmentally compromised tetraploid embryos. ES cells were capable of colonizing somatic tissues in diploid aggregation chimeras but less efficiently than ICMs of the same genotype. When ICM in equilibrium with tetraploid and ES in ...
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The first differentiation event of the mammalian embryo is thought to occur during blastulation and results in two populations of cells, the inner cell mass (ICM) and the trophectoderm. Most embryonic stem (ES) cell lines have been derived from the ICM or a further subset of ICM cells known as the epiblast. There appears to be a limited period of embryonic development during which pluripotent E...
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Analysis of experimental mouse chimeras (chimaeras) and mosaics provides a means of investigating patterning and differentiation within the developing mammalian eye. Chimeric and mosaic mice carry two or more genetically distinct cell populations and extend the repertoire of analytical tools available to the geneticist. Here we review the impact these techniques have had on our understanding of...
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Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...
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ورودعنوان ژورنال:
- Development
دوره 130 25 شماره
صفحات -
تاریخ انتشار 2003